Isozymes

Plasma alkaline phosphatase isozymes: isolation and characterization of isozymes.

I. The two isozymes of alkaline phosphatase (orthophosphoric monoester phosphohydrolase, EC 3.1.3.1) detectable in human blood group O plasma have been separated from one another and isolated in a high state of purity. With p-nitrophenyl phosphate as substrate, the catalytic properties of the two isozymes appear to be the same with respect to pH optimum, lO.5; Kin, o.35-o.4 mM, and activity in ...

Lactic Dehydrogenase Isozymes

Lactic dehydrogenase isozymes (I-lactate, NAD oxidoreductase), which are key enzymes in glycolysis, have been the object of extensive investigation. Five LDH isozymes have been found. The five isozymes represent the five possible tetramaric combinations of the two polypeptides. Thus, the five tetramers range from LDH-l( H4 ) to LDH -5 ( M4 ), the three hybrid enzymes being H3MI, H2 M2, and H j ...

Isozymes: Methods and Applications

Isozymes: Application for Population Genetics

Electrophoresis as such has been applied for many purposes, among these, analysis of isozymes or isoenzymes. As the designation indicates, this is a group of enzymes having the same catalytic ability, i.e. they catalyse the same chemical reaction. Due to the fact that enzymes are proteins and built from amino acids, they possess an electric change so isozymes are enzymes with the same catalytic...

Peroxidase Isozymes from Horseradish Roots

Seven peroxidase isozymes were isolated from horseradish roots and purified to homogeneity as ascertained by chromatography, ultracentrifugation, and polyacrylamide disk electrophoresis. Purification procedures included ammonium sulfate precipitation and column chromatography on carboxymethyl and diethylaminoethyl cellulose. Peroxidase activity in the seven isozymes accounted for 86% of the act...